Real-time PCR instrument ABI Prism 7000 Sequence Detection System ABI Prism 7700 Sequence Detection System ABI Prism 7900HT Sequence Detection System Applied Biosystems 7300 Real-Time PCR System Recommended ROX concentration 1x 1x 1x 1x…
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fluorescent signal intensities among wells when used with real time PCR instruments that have option. For Applied Biosystems 7300 Real-Time PCR System and StepOnePlus Real-Time PCR System, use of ROX Reference Dye (50X) is recommended. For Applied Biosystems 7500/7500 Fast Real-Time PCR System, the use of ROX Reference Dye II is recommended.
